Partial Inverse PCR: A Technique for Cloning Flanking Sequences
نویسندگان
چکیده
منابع مشابه
Modified inverse PCR method for cloning the flanking sequences from human cell pools.
Inverse polymerase chain reaction (PCR) is a powerful approach for cloning the DNA sequences lying outside the boundaries of known sequences (7). This approach can be used to determine the integration site of a virus or a transposon (2,4). The procedure described by Triglia (7) can be directly used to amplify the flanking sequence in a plasmid or in a bacterial genome. However, it has been diff...
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The insertion position of exogenous genes in plant genomes is usually identified by adapter ligation-mediated polymerase chain reaction (PCR), thermal asymmetric interlaced PCR, and restriction site extension PCR in transgenic plant research. However, these methods have various limitations, such as the complexity of designing primers and time-consuming and multiple-step procedures. The goal of ...
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The tandem ligation of DNA sequences within plasmid vectors is hindered by the inability to force the orientation of subsequently ligated DNA fragments and the inherent instability of repeated sequences when grown in bacterial hosts. Improvements have been made in the latter by the introduction of several E. coli strains by commercial suppliers (STBL2; Life Technologies, Gaithersburg, MD, USA o...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1997
ISSN: 0736-6205,1940-9818
DOI: 10.2144/97226bm07